Developmental toxicity assay (zebrafish larvae)
More info coming soon
One-cell stage embryo CRISPR microinjections
More info coming soon
Pseudo-de novo assembly of unmapped WGS reads
More info coming soon
Gene expression microarray
Single-channel 4x44K mRNA expression array from Agilent
Protocol: gene-expression-microarray-protocol
(optional: expression-array-stripping-protocol-sscnaoh)
RT-qPCR & standard PCR
Primer Design protocol: primer-design-protocol
Nucleic acid extraction & isolation
Qiagen RNeasy and DNeasy extraction kits, in my own words…
DNA protocol: dna-extraction-protocol
RNA protocol: rna-isolation-protocol
Immunocytochemistry
More info coming soon
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