Methods

Developmental toxicity assay (zebrafish larvae)

More info coming soon

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One-cell stage embryo CRISPR microinjections

More info coming soon

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Pseudo-de novo assembly of unmapped WGS reads

More info coming soon

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Gene expression microarray

 

Single-channel 4x44K mRNA expression array from Agilent

Protocol: gene-expression-microarray-protocol

(optional: expression-array-stripping-protocol-sscnaoh)

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RT-qPCR & standard PCR

Primer Design protocol: primer-design-protocol

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Nucleic acid extraction & isolation

Qiagen RNeasy and DNeasy extraction kits, in my own words…

DNA protocol: dna-extraction-protocol

RNA protocol: rna-isolation-protocol

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Immunocytochemistry

More info coming soon

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